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Mark C Fernandez, Glenna J Peterson, Chris Le, Shawn J Skerrett, Thomas R Hawn, Interferon Gamma Stimulation Fails to Restrict Mycobacterium tuberculosis Growth in Human Monocyte-derived and Alveolar Macrophages, The Journal of Infectious Diseases, 2025;, jiaf220, https://doi.org/10.1093/infdis/jiaf220
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Abstract
We examined whether IFNG restricts Mycobacterium tuberculosis (Mtb) growth in human macrophages across a range of conditions and methods. We observed an IFNG-dependent enhancement of bacterial replication in MCSF-differentiated monocyte-derived macrophages (MDMs, 4.84 x 105 CFU IFNG stimulated versus 2.48 x 105 CFU untreated, P < 0.001) with four Mtb strains. Mtb replication was not restricted by IFNG treatment of alveolar macrophages. In agreement with previous studies, IFNG-stimulated murine bone marrow derived macrophages effectively restricted Mtb replication. These data suggest that Mtb resists IFNG-stimulated immunity within human macrophages with implications for distinct host species immune responses.