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Silvia-Simona Grosu-Tudor, Iulia-Roxana Angelescu, Alexandru Brînzan, Medana Zamfir, Characterization of S-layer proteins produced by lactobacilli isolated from Romanian artisan fermented products, Journal of Applied Microbiology, Volume 134, Issue 1, January 2023, lxac063, https://doi.org/10.1093/jambio/lxac063
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Abstract
To characterize S-layer proteins produced by four lactobacilli isolated from Romanian artisan fermented products.
Four lactobacilli strains have been shown to produce S-layer proteins, both under optimal and stressfull conditions. The presence of S-layer proteins was confirmed by transmission electron microscopy. Removal of S-layer proteins caused a loss of the bacterial resistance to stress conditions and of the autoaggregation ability. Liquid chromatography–mass spectrometry analysis identified peptides corresponding to Slp M sequence in case of Levilactobacillus brevis 403, and peptides corresponding to Slp A sequence in case of Lactobacillus helveticus 34.9. The analysis confirmed molecular masses of ∼51 and 48 kDa, respectively, for the two proteins, and gave information about their pI, of about 9.4–9.6. A specific PCR amplification was obtained for the genome of Lact. helveticus 34.9 with slpA primers, and the amplicon sequence was 95.31% identical to slpA gene.
Our findings indicate that certain environmental stress conditions can induce the S-layer production, which helps the producing cells to survive under unfavorable conditions.
