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Pilar Villalón, Sylvia Valdezate, Maria J. Medina-Pascual, Gema Carrasco, Ana Vindel, Juan A. Saez-Nieto, Epidemiology of the Acinetobacter-derived cephalosporinase, carbapenem-hydrolysing oxacillinase and metallo-β-lactamase genes, and of common insertion sequences, in epidemic clones of Acinetobacter baumannii from Spain, Journal of Antimicrobial Chemotherapy, Volume 68, Issue 3, March 2013, Pages 550–553, https://doi.org/10.1093/jac/dks448
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Abstract
To study the distribution, diversity and activity of Acinetobacter-derived cephalosporinase (ADC)-, carbapenem-hydrolysing oxacillinase (CHO)- and metallo-β-lactamase (MBL)-encoding genes, and of the most common insertion sequences (ISs), in the genome of nosocomial, epidemic, multidrug-resistant Acinetobacter baumannii (MDRAB) clones from Spain.
The studied population included 59 MDRAB strains previously genotyped by PFGE and multilocus sequence typing. The search for the ADC (blaADC), CHO (blaOXA-51-like, blaOXA-23-like, blaOXA-40-like and blaOXA-58-like) and MBL (blaIMP, blaVIM, blaSIM-1, blaGIM-1, blaSPM-1 and blaNDM-1) genes, and for the ISs (ISAba1, ISAba2, ISAba3, ISAba4 and IS18) was done by PCR assays. The phenotypic presence of MBL enzymes was examined using imipenem/imipenem + EDTA strips.
The most prevalent IS, ISAba1 (93.2%), was detected upstream of blaADC and blaOXA-51-like. These genes showed ample diversity (10 and 8 alleles, respectively). Four ADC sequences (ADC-1-likeP240S, ADC-2-likeN260H/T264N, ADC-11-likeQ163K and ADC-11-likeG342R) are described here for the first time. blaOXA-58-like was carried by 20.3% of strains, in association with ISAba2, ISAba3 or IS18. blaOXA-40-like was the most prevalent acquired CHO gene (57.6%), and was associated with none of the studied ISs. Neither blaOXA-23-like nor ISAba4 was detected in any strain. Some 67.8% of strains with MBL activity showed no corresponding gene in PCR; these results were more common in strains with a highly active CHO, such as OXA-40.
All the studied genes and their related ISs showed a clonal distribution. Imipenem resistance was probably provided by OXA-40 for the most part, while MBL- and OXA-23-encoding genes were absent in the studied population.
- alleles
- phenotype
- polymerase chain reaction
- carbapenem
- epidemiology
- acinetobacter
- cephalosporinase
- clone cells
- edetic acid
- electrophoresis, gel, pulsed-field
- genes
- genome
- imipenem
- injury severity score
- microscopy, scanning probe
- spain
- enzymes
- edetate disodium
- epidemics
- acinetobacter baumannii
- beta-lactamase ndm-1
- idiopathic short stature