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N. J. Liptrott, P. Curley, D. Moss, D. J. Back, S. H. Khoo, A. Owen, Interactions between tenofovir and nevirapine in CD4+ T cells and monocyte-derived macrophages restrict their intracellular accumulation, Journal of Antimicrobial Chemotherapy, Volume 68, Issue 11, November 2013, Pages 2545–2549, https://doi.org/10.1093/jac/dkt225
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Abstract
There is no pharmacokinetic interaction between tenofovir and nevirapine, but a higher emergence rate of resistance mutations has been reported when these drugs are coadministered. We sought to examine if there is a potential intracellular interaction that may account for the emergence of resistant virus.
Primary CD4+ and CD14+ cells were isolated from healthy volunteer blood. Monocyte-derived macrophages were differentiated from CD14+ cells. Accumulation of radiolabelled tenofovir and nevirapine was then assessed in these cells.
We show here that tenofovir and nevirapine immune cell intracellular concentrations are lower when coincubated in CD4+ cells and monocyte-derived macrophages, but not in CD14+ cells.
These data indicate a potential intracellular drug–drug interaction between these drugs that warrants further investigation.