-
Views
-
Cite
Cite
Wen'en Liu, Lamei Chen, Hongling Li, Huili Duan, Yunli Zhang, Xianghui Liang, Xian Li, Mingxiang Zou, Li Xu, Peter M. Hawkey, Novel CTX-M β-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha, Southern China, Journal of Antimicrobial Chemotherapy, Volume 63, Issue 5, May 2009, Pages 895–900, https://doi.org/10.1093/jac/dkp068
- Share Icon Share
Abstract
The aim of this study was to undertake a survey of the occurrence of CTX-M and SHV extended-spectrum β-lactamase (ESBL) genotypes in Enterobacteriaceae from Hunan Province, China.
Clinical isolates (425) from three major hospitals in Changsha, Hunan Province, were collected between October 2004 and July 2005, and their antimicrobial susceptibilities of the genotype of blaCTX-M and blaSHV were determined. Random amplified polymorphic DNA was used to characterize the clonality of all of the isolates.
The overall rate of ESBL-positive isolates was 33.4% (142/425). The dominant ESBLs were CTX-M types, and were found in 109/142 (76.8%) isolates comprising seven different genera/species, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii, Proteus vulgaris and Providencia stuartii. The most common blaCTX-M genotypes were blaCTX-M-14 (47.7%), blaCTX-M-3 (29.4%) and blaCTX-M-15 (17.4%). A novel gene derived from blaCTX-M-15, blaCTX-M-82 (Ala-40→Pro), was identified.
The dominant ESBL genotype in Hunan Province was blaCTX-M. The high prevalence (17.4%) of blaCTX-M-15 has not previously been reported from China. Our results identify that an epidemic of blaCTX-M in Changsha, Hunan Province, has evolved with the appearance and spread of blaCTX-M-15 against the dominant genotypes blaCTX-M-14 and blaCTX-M-3. The worldwide dominance of blaCTX-M-15 could be poised to spread to China, displacing the current prevailing genotypes.