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Yasuyoshi Kamiya, Yasushi Shimada, Shin Ito, Mina Kikuchi, Mitsuru Yasuda, Yoshiaki Kawamura, Takashi Deguchi, Analysis of the quinolone-resistance determining region of the gyrA gene and the analogous region of the parC gene in Ureaplasma parvum and Ureaplasma urealyticum detected in first-void urine of men with non-gonococcal urethritis, Journal of Antimicrobial Chemotherapy, Volume 68, Issue 2, February 2013, Pages 480–482, https://doi.org/10.1093/jac/dks417
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Sir,
Many reports have suggested that Ureaplasma parvum and/or Ureaplasma urealyticum may be associated with urogenital infections, infertility, and adverse pregnancy outcomes.1 However, their pathogenic roles have remained somewhat controversial. As for the management of the ureaplasma infections, there have been only a limited number of reports on surveillance for antimicrobial resistance in ureaplasma clinical strains, which is crucial for providing therapy empirically.
Target enzymes of fluoroquinolones, DNA gyrase and topoisomerase IV, are composed of two GyrA and two GyrB subunits and two ParC and two ParE subunits, respectively. The central mechanism of fluoroquinolone resistance involves amino acid changes in GyrA and/or ParC. Fluoroquinolone resistance-associated mutations are localized in the quinolone-resistance determining region (QRDR) of the gyrA gene and the analogous region of the parC gene.2 We performed a study in which we amplified these regions from ureaplasma DNAs taken from urine specimens of men with non-gonococcal urethritis (NGU) and then determined their sequences to assess the fluoroquinolone resistance of clinical strains of U. parvum and U. urealyticum.