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Rinse K. Weersma, Lu Zhou, Ilja M. Nolte, Gerrit van der Steege, Hendrik M. van Dullemen, Elvira Oosterom, Lisette Bok, Maikel P. Peppelenbosch, Klaas N. Faber, Jan H. Kleibeuker, Gerard Dijkstra, Runt-related transcription factor 3 is associated with ulcerative colitis and shows epistasis with solute carrier family 22, members 4 and 5, Inflammatory Bowel Diseases, Volume 14, Issue 12, 1 December 2008, Pages 1615–1622, https://doi.org/10.1002/ibd.20610
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Inflammatory bowel disease (IBD), comprising Crohn's disease (CD) and ulcerative colitis (UC), are intestinal inflammatory disorders with a complex genetic background. Mice deficient for the runt-domain-transcription-factor3 (Runx3) develop spontaneous colitis. Human RUNX3 resides in an IBD-susceptibility locus. We studied the association of RUNX3 in a cohort of IBD patients and analyzed the interaction with SLC22A4/5. RUNX3 and OCTN1 mRNA expression was assessed in inflamed and noninflamed mucosa from patients and controls.
543 IBD patients (309 CD / 234 UC) and 296 controls were included. Four single nucleotide polymorphisms (SNPs) and 4 microsatellite markers were studied for RUNX3. Five SNPs (including SNP-207G→C and SNP1672C→T) were analyzed for SLC22A4/5. RUNX3, and OCTN1 expression in mucosal tissue from 30 patients (14 UC / 16 CD) and 6 controls were determined by quantitative polymerase chain reaction.
A significant association between RUNX3-SNP rs2236851 and UC (OR 1.61; 95% confidence interval [CI] 1.11–2.32, P = 0.020) was found. Carriership is associated with pancolitis (odds ratio [OR] 1.86; 95% CI 1.08–3.21). SLC22A4/5-SNPs rs272893 and rs273900 are associated with CD (OR 2.16; 95% CI 1.21–3.59 and OR 2.40; 95% CI 1.43–4.05). We found epistasis for carriership of a risk-associated allele in RUNX3 and SLC22A4/5 for UC patients versus CD patients (OR 3.83; 95% CI 1.26–11.67). RUNX3 mRNA expression is increased (P = 0.01) in inflamed colonic mucosa of UC patients compared to noninflamed mucosa and controls.
We provide evidence for the genetic association of RUNX3 with UC and for CD with the IBD5 locus including SLC22A4/5. An epistatic effect of RUNX3 and SLC22A4 was associated with an increased risk for UC. Our data suggest a role for RUNX3 in UC susceptibility.
- alleles
- polymerase chain reaction
- signal transduction
- crohn's disease
- inflammatory bowel disease
- ulcerative colitis
- cations
- colitis
- intestines
- membrane transport proteins
- microsatellite repeats
- single nucleotide polymorphism
- rna, messenger
- genetics
- mice
- mucous membrane
- transcription factor
- solutes
- octn1 protein, human
- pancolitis
- colonic mucous membrane