Volume 366, Issue 4, February 2019
Biotechnology and Synthetic Biology
Stable and efficient delivery of DNA to Bacillus subtilis (natto) using pLS20 conjugational transfer plasmids
Efficient gene delivery method to B. natto was established and the system should generate engineered B. natto strains for Microbiology and/or Biotechnology.
Environmental Microbiology
Bacterial community and metabolome shifts in the cecum and colon of captive sika deer (Cervus nippon) from birth to post weaning
Developmental shifts of bacterial community and metabolites in hindgut of sika deer.
Seasonal changes in luminescent intestinal microflora of the fish inhabiting the Bering and Okhotsk seas
Two luminescent psychrophilic bacteria species A. logei and P. phosphoreum are common for fish intestine microflora in the Bering and Okhotsk seas and supersede each other in season dependent manner.
Effect of polyhexamethylene biguanide functionalized silver nanoparticles on the growth of Staphylococcus aureus
Ag NPs-PHMB caused cell wall damage, decreased membrane fluidity, resulted in leakage of K+, Mg2+, ATP and proteins from the cell, and inhibited DNA replication, eventually leading to the loss of viability and death of S. aureus.
Food Microbiology
The potential of pectin to impact pig nutrition and health: feeding the animal and its microbiome
High frequency of coagulase-positive staphylococci carriage in healthy wild boar with detection of MRSA of lineage ST398-t011
Wild boar frequently carry coagulase-positive-staphylococci (S. aureus, S. pseudintermedius and S. hyicus), specially relevant are S. aureus (both methicillin-susceptible and methicillin-resistant) of emergent lineages of interest in human and animal medicine.
Salmonella enterica serovar Typhimurium ATCC 14028S is tolerant to plant defenses triggered by the flagellin receptor FLS2
This study demonstrates that FLS2-mediated plant defenses are ineffective in preventing growth of Salmonella enterica strain 14028S.
Probiotic Enterococcus faecalis AG5 effectively assimilates cholesterol and produces fatty acids including propionate
Probiotic AG5 has various health beneficial properties.
Pathogens and Pathogenicity
Resveratrol controls Escherichia coli growth by inhibiting the AcrAB-TolC efflux pump
Escherichia coli viability was inhibited by resveratrol through decrease of tolC-promoter activity and tolC expression resulting in significant inhibition of the AcrAB-TolC pump.
Immunogenicity characterization of genetically fused or chemically conjugated heat-stable toxin toxoids of enterotoxigenic Escherichia coli in mice and pigs
The immunogenicity of two antigen strategies, genetic fusion and chemical conjugation from STa, were comparatively investigated to prepare safe and immunogenic STa antigens for ETEC vaccine development.
Vaginal microbiota transplantation for the treatment of bacterial vaginosis: a conceptual analysis
Vaginal microbiota transplantation: an a effective way to treat bacterial vaginosis?
Physiology and Biochemistry
Golden Gate-based metabolic engineering strategy for wild-type strains of Yarrowia lipolytica
The Golden Gate-derived Multiple Organism Cloning System (GoldenMOCS) is extended to the yeast Y. lipolytica including CRISPR/Cas9 technology.
Virology
Modification of T2 phage infectivity toward Escherichia coli O157:H7 via using CRISPR/Cas9
The possibility of artificial alternation of host specificity of bacteriophage, which is important for therapeutic application of bacteriophage, was explored by CRISPR/Cas, the newly developed tool of genetic engineering.
Incubator
Molecular host mimicry and manipulation in bacterial symbionts
This mini-review explores recent research on how mutualistic bacteria, in addition to pathogenic bacteria, manipulate host cells by using proteins that mimic eukaryotic factors.
