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Summary

A method by which non-cultivable filamentous segmented microbes colonising the ileal epithelium of mice can be harvested was devised. Intact epithelial cells with attached filamentous microbes were obtained. An ethanol-treated preparation of epithelial material was used to restore the filamentous segmented microbes to the normal microflora of mice maintained by gnotobiotic methodology.

Epithelium, Discontinuous density gradient centrifugation

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© 1987 Federation of European Microbiological Societies
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