Abstract

The production of urea by Thiosphaera pantotropha was studied. Batch cultures were grown on acetate as energy source and with NO3 or O2 as terminal electron acceptor. Urea accumulated in the media during exponential growth in aerobic and anaerobic cultures of T. pantotropha. Urea production continued after the cells had entered the stationary growth phase. Bacterial ability to produce urea was supported by studies of cultures enriched for denitrifying, sulphate-reducing and fermenting bacteria. The results implied that urea production was common among bacteria normally considered to be important in marine sediments.

References

1

Eppley
R.W.
Renger
E.H.
Venrick
E.L.
Mullin
M.M.
(
1973
)
A study of plankton dynamics and nutrient cycling in the central gyre of the North Pacific Ocean
Limnol. Oceanogr.
,
18
,
534
551
.

2

Harrison
W.G.
Head
E.J.H.
Conover
R.J.
Longhurst
A.R.
Sameoto
D.D.
(
1985
)
The distribution and metabolism of urea in the eastern Canadian Arctic
Deep-Sea Res.
,
32
,
23
42
.

3

Kaufman
Z.G.
Lively
J.S.
Carpenter
E.J.
(
1983
)
Uptake of nitrogenous nutrients by phytoplankton in a barrier island estuary: Great South Bay, New York
Estuar. Coast. Shelf Sci.
,
17
,
483
493
.

4

Kristiansen
S.
(
1983
)
Urea as a nitrogen source for the phytoplankton in the Oslofjord
Mar. Biol.
,
74
,
17
24
.

5

Kristiansen
S.
Lund
B.A.
(
1989
)
Nitrogen cycling in the Barents Sea-I. Uptake of nitrogen in the water column
Deep-Sea Res.
,
36
,
255
268
.

6

McCarthy
J.J.
(
1972
)
The uptake of urea by natural populations of marine phytoplankton
Limnol. Oceanogr.
,
17
,
738
748
.

7

McCarthy
J.J.
Taylor
W.R.
Taft
J.L.
(
1977
)
Nitrogenous nutrition of the plankton in the Chesapeake Bay. 1. Nutrient availability and phytoplankton preferences
Limnol. Oceanogr.
,
22
,
996
1011
.

8

Remsen
C.C.
(
1971
)
The distribution of urea in coastal and oceanic waters
Limnol. Oceanogr.
,
16
,
732
740
.

9

Remsen
C.C.
Carpenter
E.J.
Schroeder
B.W.
(
1974
)
The role of urea in marine microbial ecology
In
Effects of the Ocean Environment on Microbial Activities
Colwell
R.R.
Morita
R.Y.
, Eds) pp
286
304
University Park Press
,
Baltimore
.

10

Berman
T.
(
1974
)
Urea in the waters of Lake Kinneret (Sea of Galilee)
Limnol. Oceanogr.
,
19
,
977
980
.

11

Satoh
Y.
Okino
T.
Aoyama
K.
(
1980
)
Correlation between urea and other chemical and biological parameters in waters of Lake Suwa
Japan. Int. Revue ges. Hydrobiol.
,
65
,
445
454
.

12

McCarthy
J.J.
Kamykowski
D.
(
1972
)
Urea and other nitrogeneous nutrients in La Jolla Bay during February, March and April 1970
Fish. Bull.
,
70
,
1261
1274
.

13

Blackburn
T.H.
(
1987
)
Microbial food webs in sediments
In
Microbes in the Sea
Sleight
M.A.
, Ed) pp
39
58
Ellis Horwoos Limited
,
Chichester
.

14

Lomstein
B.Aa.
Blackburn
T.H.
Henriksen
K.
(
1989
)
Aspects of nitrogen and carbon cycling in the northern Bering Shelf sediment. I. The significance of urea turnover in the mineralization of NH4+
Mar. Ecol. Prog. Ser.
,
57
,
237
247
.

15

Lomstein
B.Aa.
Blackburn
T.H.
(
1992
)
Sediment nitrogen cycling in Aarhus Bay, Denmark
The Danish Environmental Protection Agency
Copenhagen
.

16

Therkildsen
M.S.
Lomstein
Aa.L.
(
1993
)
Seasonal variation in sedimental urea turnover in a shallow estuary
Mar. Ecol. Prog. Ser.
, submitted.

17

Vogels
G.D.
Drift
C.
(
1976
)
Degradation of purines and pyrimidines by microorganisms
Bacteriol. Rev.
,
40
,
403
468
.

18

Pedersen
H.
Lomstein
B.Aa.
Blackburn
T.H.
(
1993
)
Bacterial urea production in marine sediment
FEMS Microbiol. Ecol.
,
12
,
51
59
.

19

Vishniac
W.
Santer
M.
(
1957
)
The Thiobacilli
Bacteriol Rev.
,
21
,
195
213
.

20

Price
N.M.
Harrison
P.J.
(
1987
)
Comparison of methods for the analyses of dissolved urea in seawater
Mar. Biol.
,
94
,
307
317
.

21

Baudinet
D.
Galgani
F.
(
1991
)
A new application of microplate reader in oceanography: Colorimetric assays to determine some nitrogen forms
Estuar. Coast. Shelf Sci.
,
33
,
459
466
.

22

Lund
B.Aa.
Blackburn
T.H.
(
1989
)
Urea turnover in a coastal marine sediment measured by a 14C-urea short-term incubation
J. Microbiol. Methods
,
9
,
297
308
.

23

Widdel
F.
Bak
F.
(
1992
)
Gram-negative mesophilic sulfate-reducing bacteria
In
The Procaryotes
Balows
H.
Trüper
H.G.
Dwarkin
M.
Harder
W.
Schleifer
H.
, Eds) 2nd edn., pp
3352
3378
Springer Verlag
,
New York
.

24

Morris
D.R.
Koffron
K.L.
(
1967
)
Urea production and putrescine biosynthesis by Escherichia coli
J. Bacteriol.
,
94
,
1516
1519
.

25

Satoh
Y.
(
1980
)
Production of urea by bacterial decomposition of organic matter including phytoplankton
Int. Revue ges. Hydrobiol.
,
65
,
295
301
.

26

Hjort
U.
Kating
H.
(
1966
)
Untersuchungen zum stoffwechsel des harnstoffs bei mikroorganismen. IV. Adaptive ureasbildung bei Micrococcus denitrificans Beij.
Arch. Mikrobiol.
,
54
,
177
183
.

27

Hjort
U.
Kleczkowski
K.
Kating
H.
(
1967
)
Untersuchung zum stoffwechsel des harnstoffs in mikroorganismen. VI. Die spezifischen aktivitäten der enzyme des ornithin-cyclus in Micrococcus denitrificans Beij.
Arch. Mikrobiol.
,
55
,
311
319
.

28

Gruninger
S.E.
Goldman
M.
(
1988
)
Evidence for urea cycle activity in Sporocarcina ureae
Arch. Microbiol.
,
150
,
394
399
.

29

Jahn
O.
Sauerstein
J.
Reuter
G.
(
1987
)
Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin
Arch. Microbiol.
,
147
,
174
178
.

30

Poolman
B.
Driessen
A.J.M.
Konings
W.N.
(
1987
)
Regulation of arginine-ornithine exchange and the arginine deiminase pathway in Streptococcus lactis
J. Bacteriol.
,
169
,
5597
5604
.

31

Ramaley
R.F.
Bernlohr
R.W.
(
1965
)
Apparent induction of ornithine transcarbamylase and arginase by arginine in Bacillus Licheniformis
J. Mol. Biol.
,
11
,
842
844
.

32

Stalon
V.
Wauven
C.V.
Momin
P.
Legrain
C.
(
1987
)
Catabolism of arginine, citrulline and ornithine by Pseudomonas and related bacteria
J. Gen. Microbiol.
,
133
,
2487
2495
.

33

Robertson
L.A.
Kuenen
J.G.
(
1983
)
Thiosphaera pantotropha gen. nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium
J. Gen. Microbiol.
,
129
,
2847
2855
.

34

Jahns
T.
Zobel
A.
Kleiner
D.
Kaltwasser
H.
(
1988
)
Evidence for carrier-mediated, energy-dependent uptake of urea in some bacteria
Arch. Microbiol.
,
149
,
377
383
.

35

Kaltwasser
H.
Krämer
J.
Conger
W.R.
(
1972
)
Control of urease formation in certain aerobic bacteria
Arch. Mikrobiol.
,
178
,
178
196
.

36

McLean
R.J.C.
Nickel
J.C.
Cheng
K.J.
Costerton
J.W.
(
1988
)
The ecology and pathogenicity of urease-producing bacteria in the urinary tract
CRC Crit. Rev. Microbiol.
,
16
,
37
79
.

37

Zorn
C.
Dietrich
R.
Kaltwasser
H.
(
1982
)
Regulation by repression af urease biosynthesis in Proteus rettgeri.
Z. allg. Mikrobiol.
,
22
,
197
203
.

38

Jahns
T.
(
1992
)
Urea uptake by the marine bacterium Deleya venusta HG1
J. Gen. Microbiol.
,
138
,
1815
1820
.

39

Robertson
L.A.
Kuenen
J.G.
(
1984
)
Aerobic denitrification: a controversy revived
Arch. Microbiol.
,
139
,
353
354
.

40

Jensen
K.M.
Cox
R.P.
(
1992
)
Effects of sulfide and low redox potential on the inhibition of nitrous oxide reduction by acetylene in Pseudomonas nautica
FEMS Microbiol. Lett.
,
96
,
13
18
.

41

McLean
R.J.C.
Nickel
J.C.
Cheng
K.J.
Costerton
J.W.
(
1988
)
The ecology and pathogenicity of urease-producing bacteria in the urinary tract
CRC Crit. Rev. Microbiol.
,
16
,
37
79
.

42

Gibbons
R.J.
Doetsch
R.N.
(
1959
)
Physiological study of an obligately anaerobic ureolytic bacterium
J. Bacteriol.
,
77
,
417
428
.

43

Ladd
J.N.
Jackson
R.B.
(
1982
)
Biochemistry of ammonification
In
Agronomy no. 22
Stevenson
F.J.
, Ed) pp
173
228
American Society of Agronomy, Inc
,
Madison, Wisconsin
.

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