Abstract

In the course of our experiments with the determination of dehydroepiandrosterone (DHEA) (Oertel & Eik-Nes, 1958), various enzymes have been tried for an efficient cleavage of DHEA conjugates present in blood plasma. Only a small fraction of DHEA in blood is conjugated with glucuronic acid (Migeon, 1954). Judged from the levels of DHEA in plasma seen after acid hydrolysis, DHEA could, however, be present in a sulfate form, since DHEA sulfate has been isolated from human urine (Munson et al., 1944).

In the present paper the cleavage of DHEA conjugates in human blood plasma by means of acid and alkaline phosphatase is reported.

Methods

Acid phosphatase and semi-purified alkaline phosphatase were obtained from Worthington Biochemical Corp., β-glucuronidase from Warner-Chilcott Co., and »Glusalase« from the Endo-Laboratories.

Ethanol extracts were prepared from pooled human plasma as described in our previous paper. After drying, the residue was distributed between 50 % aqueous methanol and

This content is only available as a PDF.
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://dbpia.nl.go.kr/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
You do not currently have access to this article.