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Georg W Oertel, Kristen B Eik-Nes, Dehydroepiandrosterone in Blood: I. Cleavage of dehydroepiandrosterone conjugates by phosphatases, Acta Endocrinologica, Volume 28, Issue 3, Jul 1958, Pages 293–299, https://doi.org/10.1530/acta.0.0280293
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Abstract
In the course of our experiments with the determination of dehydroepiandrosterone (DHEA) (Oertel & Eik-Nes, 1958), various enzymes have been tried for an efficient cleavage of DHEA conjugates present in blood plasma. Only a small fraction of DHEA in blood is conjugated with glucuronic acid (Migeon, 1954). Judged from the levels of DHEA in plasma seen after acid hydrolysis, DHEA could, however, be present in a sulfate form, since DHEA sulfate has been isolated from human urine (Munson et al., 1944).
In the present paper the cleavage of DHEA conjugates in human blood plasma by means of acid and alkaline phosphatase is reported.
Acid phosphatase and semi-purified alkaline phosphatase were obtained from Worthington Biochemical Corp., β-glucuronidase from Warner-Chilcott Co., and »Glusalase« from the Endo-Laboratories.
Ethanol extracts were prepared from pooled human plasma as described in our previous paper. After drying, the residue was distributed between 50 % aqueous methanol and