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Birgitte Wuyts, Joris R Delanghe, Ishmael Kasvosve, Annick Wauters, Hugo Neels, Jacques Janssens, Determination of Carbohydrate-deficient Transferrin Using Capillary Zone Electrophoresis, Clinical Chemistry, Volume 47, Issue 2, 1 February 2001, Pages 247–255, https://doi.org/10.1093/clinchem/47.2.247
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Abstract
Background: Current methods for carbohydrate-deficient transferrin (CDT) often suffer from low precision, complexity, or risk of false positives attributable to genetic variants. In this study, a new capillary zone electrophoresis (CZE) method for CDT was developed.
Methods: CZE was performed on a P/ACE 5000 using fused-silica capillaries [50 μm (i.d.) × 47 cm] and the CEOFIX CDT buffer system with addition of 50 μL of anti-C3c and 10 μL of anti-hemoglobin. Native sera were loaded by high-pressure injection for 3 s, separated at 28 kV over 12 min, and monitored at 214 nm.
Results: CDT was completely resolved by differences in migration times (di-trisialotransferrin, 9.86 ± 0.05 min; monosialotransferrin, 9.72 ± 0.05 min; asialotransferrin, 9.52 ± 0.04 min), with a CV of 0.15%. The number of theoretical plates was 312 000 ± 21 000 for the mono- and 199 000 ± 6500 for the di-trisialylated transferrin. Genetic CB and CD variants showed prominent peaks with migration times of 10.12 ± 0.06 and 9.89 ± 0.03 min, respectively, and the carbohydrate-deficient glycoprotein syndrome could be detected, excluding false-positive results. CZE results (as a percentage; y) correlated with the Axis %CDT TIATM (x) values by Deming regression analysis: y = 1.92x − 7.29; r = 0.89. CDT values in 130 healthy nonalcoholics were determined. The 2.5th and 97.5th percentiles were 1.84% and 6.79%.
Conclusions: CZE without sample pretreatment can determine CDT with good precision, allows detection of variants, and correlates with ion-exchange chromatography.
