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D Roche, V Atger, N T Le Quang, A Girard, O G Ekindjian, Polyacrylamide gel electrophoresis in quantification of high-density lipoprotein cholesterol., Clinical Chemistry, Volume 31, Issue 11, 1 November 1985, Pages 1893–1895, https://doi.org/10.1093/clinchem/31.11.1893
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Abstract
We evaluated a method for quantifying high-density lipoprotein cholesterol in plasma, based on electrophoretic migration of the prestained (with Sudan Black III) sample through a discontinuous polyacrylamide++ gel and densitometric integration of the stain associated with each class of lipoprotein. With this method, operations can be carried out on all types of lipoproteins over a broad range of concentrations. Overloading with very-low and low-density lipoproteins did not affect reliability within a wide range of HDL concentrations (0.45 to 16.60 mmol/L). Results for 22 individual plasma samples from normal and dyslipemic subjects correlated well with those by ultracentrifugal analysis (r=0.96; Student's t= 0.90, p > 0.30). We conclude that this method is reliable, sensitive, and accurate, It may be used for simultaneously typing dyslipoproteinemias and assaying HDL cholesterol.