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Herbert S Strickler, Judith M Petty, Patricia J Stanchak, A Facilitated Fractionation of Stable Urinary Estrogens before Their Automated Fluorometric Assay, Clinical Chemistry, Volume 17, Issue 12, 1 December 1971, Pages 1186–1190, https://doi.org/10.1093/clinchem/17.12.1186
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Abstract
Urine samples to be assayed for estrone, estradiol-17β, and estriol are processed as usual: hydrolysis, ether extraction, and saponification, as well as partition between toluene and alkali. The samples are purified further, fractionated, and assayed by connecting columns containing Sephadex LH-20 to the sample line of an automated system for fluorometric assay. With this procedure, a sample can be fractionated and assayed every 45 min.
Sephadex chromatography, acid hydrolysis of estrogen conjugates, Celite, AutoAnalyzer, total estrogens in pregnancy urine, diagnostic aid
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© 1971 The American Association of Clinical Chemists, Inc.
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