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J. W. Almond, A. J. Cann, P. D. Minor, P. Reeve, P. Reeve, G. C. Schild, R. Hauptmann, G. Stanway, Nucleotide Sequence from Neurovirulent and Attenuated Strains of Type 3 Poliovirus, Reviews of Infectious Diseases, Volume 6, Issue Supplement_2, May-June 1984, Pages S487–S493, https://doi.org/10.1093/clinids/6.Supplement_2.S487
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Abstract
As part of an investigation into the molecular basis of attenuation in the Sabin poliovirus vaccines, the genomes of three strains of type 3 poliovirus differing in neurovirulence were cloned in Escherichia coli. The nucleotide sequence of the region of the genome encoding the virion protein 1 (VPl) polypeptide from each of these strains is presented. Very few changes were observed, a finding suggesting that the mutation(s) involved in the attenuation of type 3 poliovirus probably lies elsewhere in the genome. The complete nucleotide sequence of one of the strains, P3/Sabin, has been obtained and is compared with the published sequences of type 1 poliovirus. Between serotypes 1 and 3, there is 77% homology in nucleotide sequence and 90% homology in the amino acid sequence predicted for viral proteins.