Abstract

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), created an urgent need for effective antiviral treatments. While Remdesivir (GS-5734) and its parent nucleoside GS-441524 have been explored as anti-SARS-CoV-2 agents, their use is limited by toxicity concerns. Here we synthesized a 4′-thiomodified derivative of GS-441524 () using a refined C-glycosylation strategy. In VeroE6 cells infected with SARS-CoV-2, demonstrated modest antiviral activity without detectable cytotoxicity. Although its potency was lower than Remdesivir, its favorable safety profile suggests has potential as a safer antiviral for RNA viruses.

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