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Katsuo Koshi, Yasunori Suzuki, Yuki Nakaya, Kei Imai, Misa Hosoe, Toru Takahashi, Keiichiro Kizaki, Takayuki Miyazawa, Kazuyoshi Hashizume, Bovine Trophoblastic Cell Differentiation and Binucleation Involves Enhanced Endogenous Retrovirus Element Expression., Biology of Reproduction, Volume 87, Issue Suppl_1, 1 August 2012, Page 379, https://doi.org/10.1093/biolreprod/87.s1.379
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Endogenous retrovirus (ERV) envelope (env) genes are involved in the differentiation of trophoblastic cells in humans and mice. However, there is limited information about their roles in ruminant trophoblastic cells. The purpose of this study is to explore the possible roles of ERV elements in the binucleation of bovine trophoblastic cells using three in vitro bovine trophoblastic (BT) cell lines, BT-1, BT-C and BT-K. On-Matrigel culture was used to induce binucleate cells (BNC) in the BT cell lines. During induction of BNC culture, the expression profiles of ERV elements (bERVE-A, bERVE-B, BERV-K1 env and BERV-K2 env) were examined by a quantitative RT-PCR (qRT-PCR) and immunocytochemistry, and BNC specific genes (bCSH1, bPRP-1 and bPAG1) were examined for the confirmation of binucleation. The binucleation was found in BT-1 and BT-C but not in BT-K during on-Matrigel culture, i.e., the BNC specific genes were confirmed more than 10-times by qRT-PCR. The increment of bPRP-1 expression cell numbers was found in BT-C immunocytochemically. The expression intensities of bERVE-A and BERV-K1 env were increased in BT-1 and BT-C but not in BT-K during on-Matrigel culturing, and also the cell number of BERV-K1 Env protein expressed was increased in BT-C. The expression levels of these ERV elements in BT cells were consistent with those of BNC specific genes during on-Matrigel culturing (P < 0.01). These results suggest that bERVE-A and BERV-K1 env are closely involved in the expression of BNC specific genes. The on-Matrigel culture system using BT cell lines supplies a convenient in vitro tool for studying bovine trophoblastic cell lineages. This study was supported by JSPS (Kiban-kenkyu B 23380162 and Bilateral Program).
