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Chi Hun Park, Young Hee Jeong, Sang-Goo Lee, Yeun Ik Jeong, Eui-Bae Jeung, Sang-Hwan Hyun, Taeyoung Shin, Woo Suk Hwang, Sexual Dimorphic Expression of X-linked Genes in Individual Female and Male In Vivo and Cloned Porcine Blastocysts., Biology of Reproduction, Volume 87, Issue Suppl_1, 1 August 2012, Page 201, https://doi.org/10.1093/biolreprod/87.s1.201
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To elucidate the effect on reprogramming efficiency during porcine nuclear transfer we analyzed the transcription of X-linked (XIST, G6PD, HPRT1, PGK1, ZXDA and BEX1) genes in in vivo and cloned blastocysts. Our result clearly shows that sex-dimorphic expression in X-linked genes between female and male in vivo blastocysts. The expression of XIST, G6PD, HPRT1, PGK1 and BEX1 was significantly higher in female than in male blastocysts. There was, on contrary, ZXDA display higher levels in male than in female blastocysts. The results show that XIST transcripts were significantly different between female and male cloned blastocysts as seen in vivo ones. The majority of X-lined genes tested revealed aberrant expression patterns in most of cloned blastocysts. There was marked heterogeneity in XIST and PGK1 transcript levels among individual cloned blastocysts, especially in males. Moreover, these inappropriate expression patterns in cloned embryos were not ameliorated by scriptaid treatment, although blastocyst rate remarkably increased by this treatment. These results indicate that dosage compensation between male and female porcine blastocysts is achieved at this developmental stage and altered expression of X-linked gene is frequently occurred in SCNT embryos. These observation that transcription failure in cloned embryos probably due to aberrant epigenetic patterns and faulty genome reprogramming, suggesting that the regulatory mechanisms of X-inactivation and X-linked gene expression are substantially altered in SCNT embryos.This work was supported by a grant from Next-Generation BioGreen 21 program (No. PJ008323), Rural Development Adminstration, Republic of Korea.
