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Charles A. Blake, Gary T. Campbell, Franco Mascagni, Michael D. Culler, Andres Negro-Vilar, Effects of Injection of Anti-Luteinizing Hormone (LH)-Releasing Hormone Serum and Anti-Gonadotropin-Releasing Hormone-Associated Peptide Serum into Neonatal Rats on LH and Follicle-Stimulating Hormone Cells, Biology of Reproduction, Volume 49, Issue 5, 1 November 1993, Pages 965–971, https://doi.org/10.1095/biolreprod49.5.965
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Abstract
Relatively little is known regarding the potential importance of LHRH and of gonadotropin-releasing hormone-associated peptide (GAP) on the postnatal development of gonadotrophs. We investigated the effects of administration of anti (A)-LHRH serum or A-GAP serum to neonatal rats on the development of LH and FSH immunoreactivity in anterior pituitary gland (APG) cells. Serum (sheep non-immune [NSS], sheep A-LHRH, sheep A-LHRH/GAP [which bound LHRH and GAP], rabbit non-immune [NRS], or rabbit A-GAP) was injected s.c. into neonatal female and male rats on Days 1 and 3 or Days 1, 3, 5, and 7 after birth. Pups were killed on Day 5 or 9, two days after the last injection. The percentages of APG cells immunoreactive for LH or FSH increased from Day 1 to Day 5 and did not change between Days 5 and 9 in female pups treated with NSS or NRS. There was a trend for the percentages of LH and FSH cells to increase from Day 1 to Days 5 and 9 in male pups treated with NSS or NRS, but the increases were not statistically significant. In both females and males, treatment with antisera that recognized LHRH reduced the percentage of FSH cells on Day 5 and the percentages of LH and FSH cells on Day 9. Treatment with A-GAP was without effect in both sexes. There were similar percentages of LH and FSH cells in females and a lower percentage of FSH than of LH cells in males in the Day 5 and 9 controls. In females, administration of antisera that recognized LHRH resulted in a lower percentage of FSH than of LH cells on Days 5 and 9.
LH and FSH cell size did not change from Day 1 to Day 5 or 9 in controls of either sex. In females, administration of antisera that recognized LHRH decreased the size of FSH cells on Days 5 and 9, and A-LHRH/GAP decreased the size of LH cells on Day 9. A-GAP had no effect in females, and no treatment had any significant effect on the size of LH or FSH cells in males. LH and FSH cell sizes were not different within any group of either sex.
The results indicate that LHRH but not GAP plays a role in the development of the percentage of APG cells with LH or FSH immunoreactivity in the APG of female and male rats and affects the size of gonadotrophs in female rats. The results also suggest that, at least in females, LHRH is important for maintaining FSH synthesis in gonadotrophs.
