Abstract

4-Keto-d-arabonate (d-threo-pent-4-ulosonate) and 4-keto-d-ribonate (d-erythro-pent-4-ulosonate) were prepared from d-arabinose and d-ribose by two successive reactions of membrane-bound enzymes, d-aldopentose 4-dehydrogenase and 4-keto-d-aldopentose 1-dehydrogenase of Gluconobacter suboxydans IFO 12528. Alternatively, they were prepared from d-arabonate and d-ribonate with another membrane-bound enzyme, d-pentonate 4-dehydrogenase. Analytical data confirmed the chemical structures of the 4-pentulosonates prepared. This is the first report of successful enzymatic synthesis of 4-pentulosonates.

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