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Naoko INOUE, Yoshio SUZUKI, Kenji YOKOYAMA, Isao KARUBE, Novel Fluorescent Probe for Analysis of Hydroperoxides Based on Boron Dipyrromethane Fluorophore, Bioscience, Biotechnology, and Biochemistry, Volume 73, Issue 5, 23 May 2009, Pages 1215–1217, https://doi.org/10.1271/bbb.80899
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Abstract
A new reagent, N-[2-(diphenylphosphino)ethyl]-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide (DPPEA-BODIPY), was designed and synthesized for analysis of hydroperoxides. DPPEA-BODIPY fluoresces at low levels in the visible range (λex⁄λem = 502 nm/515 nm) and reacts with hydroperoxides to produce DPPEA-BODIPY oxide, which fluoresces at high levels. The fluorescence intensity of the reaction mixture was observed to be linearly related to the methyl linoleate hydroperoxide (MeLOOH) concentration.