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Keigo HISAMATSU, Hideaki UNNO, Shuichiro GODA, Tomomitsu HATAKEYAMA, Effects of Ca2+ on Refolding of the Recombinant Hemolytic Lectin CEL-III, Bioscience, Biotechnology, and Biochemistry, Volume 73, Issue 5, 23 May 2009, Pages 1203–1205, https://doi.org/10.1271/bbb.80793
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Abstract
CEL-III is a hemolytic lectin isolated from Cucumaria echinata. Although recombinant CEL-III (rCEL-III) expressed in Escherichia coli showed very weak hemolytic activity compared with native protein, it was considerably enhanced by refolding in the presence of Ca2+. This suggests that Ca2+ supported correct folding of the carbohydrate-binding domains of rCEL-III, leading to effective binding to the cell surface and subsequent self-oligomerization.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009
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