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Takahiro OBA, Yoshitsugu YAMAMOTO, Shuji NOMIYAMA, Hikaru SUENAGA, Shigeru MUTA, Kosuke TASHIRO, Satoru KUHARA, Properties of a Trifluoroleucine-Resistant Mutant of Saccharomyces cerevisiae, Bioscience, Biotechnology, and Biochemistry, Volume 70, Issue 7, 23 July 2006, Pages 1776–1779, https://doi.org/10.1271/bbb.50640
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Abstract
We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.