Azoles exhibit binding to only one or two out of the three binding sites in the ScPdr5p binding pocket. (A) Growth inhibition zones of the PDR5+ S. cerevisiae strain exposed to azoles, benchmark substrates of individual binding sites and their respective combinations. Stock solutions of azoles: clotrimazole (4.4 mM), miconazole (3.6 mM), bifonazole (1.6 mM), ketoconazole (7.6 mM) and itraconazole (4.2 mM). Stock solutions of benchmark substrates: rhodamine 6G (10 mM), TI (1 mM) and TBT (2.8 M). Asterisks highlight combinations of azoles and benchmark substrates leading to an enlargement of the growth-inhibition zones. (B) Schematic overview of the binding sites of tested azoles, fluorescent probe diS-C3(3) and benchmark substrates in the ScPdr5p binding pocket.
