In vivo and in vitro effect of CB1R blockade and/or CB2R activation on nephrin, podocin and synaptopodin gene expression. Nephrin (A), podocin (B) and synaptopodin (C) mRNA levels were measured by real-time PCR on total RNA extracted from the renal cortex of ND mice and DM treated with AM6545, AM1241 or both (A + A) (n = 8 mice per group). *P < 0.05, DM group versus ND groups; **P < 0.05, treated DM groups versus DM group. (D) Cultured podocytes were exposed to glycated albumin (GA 200 μg/mL) for 24 h in the presence of AM1241 (10 μmol/L), AM6545 (10 μmol/L) or both (A + A), then total mRNA was extracted and nephrin mRNA levels measured by real-timePCR. *P < 0.05, GA versus others; **P < 0.05, vehicle versus treated groups.