OsCOL10 was preferentially expressed in the leaves. (A) Thirty-day-old wild-type plants grown under SDs were used for qRT-PCR. DL1, newly emerging leaf; DL2, expanding leaf; DL3, fully expanded leaf; ASA, around the shoot apex. (B) OsCOL10 transcript levels in various organs (means ± standard deviation, n = 3). (C–J) Histochemical staining of various organs in p OsCOL10 :: GUS transgenic plants. (C) Root; (D) stem; (E) transverse sections of the stem; (F) magnified image of the boxed area in (E); (G and H) transverse sections of the leaf blade and sheath; (I) enlarged image of the boxed area in (H); (J) floret. (K–L) RNA in situ hybridization analysis of OsCOL10. The materials were harvested at 4 h after dawn under LD conditions. Leaf blades of 35-day-old wild-type plants were cross-sectioned and hybridized with OsCOL10 -specific antisense (K) or sense (L) probes. x, xylem; p, phloem; ep, epidermis; m, mesophyll.