Figure 4
Differential expression of STE13ca gene in C. albicans ATCC 10231 during different growth phases, nitrogen sources and during dimorphism using RT-PCR. (a) Digital imagery of cellular morphology, (b) cDNA STE13ca gene, (c) 18S rDNA cDNA, and (d) STE13ca gene expression. (d)-I Growth phases in YEPD: (1) logarithmic, 6 h; (2) early stationary, 12 h; (3) late stationary, 24 h. (d)-II Incubation for 12 h in YNB medium with different nitrogen sources: (4) YNB, (5) YNB–proline, (6) YNB–NH2, (7) YNB–peptone. (d)-III Dimorphism in Lee's medium: (8) phase G1, (9–10) germinal tube formation, (11–12) pseudomycelium formation, (13) mycelium formation. The intensity of bands STE13ca cDNA was normalized to the intensity of 18S rDNA cDNA.

Differential expression of STE13ca gene in C. albicans ATCC 10231 during different growth phases, nitrogen sources and during dimorphism using RT-PCR. (a) Digital imagery of cellular morphology, (b) cDNA STE13ca gene, (c) 18S rDNA cDNA, and (d) STE13ca gene expression. (d)-I Growth phases in YEPD: (1) logarithmic, 6 h; (2) early stationary, 12 h; (3) late stationary, 24 h. (d)-II Incubation for 12 h in YNB medium with different nitrogen sources: (4) YNB, (5) YNB–proline, (6) YNB–NH2, (7) YNB–peptone. (d)-III Dimorphism in Lee's medium: (8) phase G1, (9–10) germinal tube formation, (11–12) pseudomycelium formation, (13) mycelium formation. The intensity of bands STE13ca cDNA was normalized to the intensity of 18S rDNA cDNA.

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