Effects of biapenem (BIA) and/or AIA-1 on in vivo PAO1 plac-lux bioluminescence signals. PAO1 plac-lux (10⁶ cfu) was inoculated into one thigh of each animal. Therapy was performed with biapenem alone or combined with AIA-1, or AIA-1 alone. (a) Representative in vivo bioluminescence in colour-scale images of thighs of mice. (b) Bacterial counts as measured by in vivo bioluminescence (photons/s) at 10 h. (c) Bacterial counts as measured by cfu at 10 h. *P < 0.05; **P < 0.01 (Student’s t-test). Error bars show SDs for three experiments. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.