Fig. 7.
Cytokinin-dependent activation of transcript elongation in vitro by CBP70 from etiolated maize seedlings. The effects of CBP and trans-zeatin (tZ) on RNA synthesis were studied in the transcription elongation system containing RNA polymerase I associated with chromatin isolated from barley leaves. The effects of the following substances added to the reaction medium (100 μl) were studied: CBP70 (20 μg), mAb (20 μg) raised against CBP70, AM, α-amanitin (4 μg ml−1), and tZ (10−7 M),