PCR amplification at an annealing temperature of 65 °C of plasmid DNA from the nid and pdo clones derived from Mycobacterium sp. S65 using primers designed specifically from the nidA gene. Lane M is a 100 bp molecular weight ladder, lanes 1–5 are the nid clone (1), the pdo clone (2), S65 (3), PYR-1 (4) and negative control (5), all amplified using the nidAF/nidAR primers, and lanes 6–10 are the nid clone (6), the pdo clone (7), S65 (8), PYR-1 (9) and negative control (10), amplified with the pdoAF/pdoAR primers. The star indicates the position of the 500 bp fragment in the molecular marker, and the arrow indicates the position of the expected amplicon.