Time course for mineralization of various PAHs by Mycobacterium sp. strain S65. Strain S65 was pre-grown in a low nutrient broth to the mid-log phase and the culture was washed with MSM, to remove residual nutrients, prior to inoculation. The ability of strain S65 to mineralize PAHs was tested on anthracene, naphthalene, fluorene, phenanthrene, and pyrene.