16S rRNA gene DGGE fingerprinting of Acinetobacter communities in soil F samples treated in a laboratory experiment using PCR products obtained with Acinetobacter specific primers GC-Ac436f and Ac676r in the direct PCR approach (a) and the nested PCR approach (b). (a) DGGE profiles for strain A. johnsonii DSM6963T (lane 1), strain A. haemolyticus LH168 (lane 2), strain A. junii DSM6964T (lane 3), soil F prior to treatment (lanes 4 and 5), soil F70MN T1 (lanes 6 and 7), soil F70HNa T1 (lanes 8 and 9), soil F70HNa T3 (lanes 10 and 11), soil F70HNa T4(lanes 12 and 13), soil F70HNa T5 (lanes 14 and 15), soil F70HNa T6 (lanes 16 and 17), soil F70HNa T9 (lanes 18 and 19). All soil samples were analyzed in duplicate. Subscripts T1, T3,… refer to sampling times as described in materials and methods. No Acinetobacter population was monitored in soil FMN. Cloned ‘bands’ are indicated within the soil fingerprint based on the comparison of the migration profiles of pure clones and the soil profile. (B) DGGE ladder (lane 1); DGGE profile of soil F70MN T1 obtained by the direct PCR (lane 2), and the nested PCR (lane 3); DGGE profile of soil F70HNa T3 obtained by the direct PCR (lane 4), and the nested PCR (lane 5); DGGE profile of soil F70MNT3 obtained by the direct PCR (lane 6), and the nested PCR (lane 7). The DGGE ladder consists of A. johnsonii, A. haemolyticus, A. lwoffii, and A. junii.
