Multiplex PCR amplification of DNA isolated from ascocarps of T. melanosporum, T. brumale and T. indicum, their relative quiescent ectomycorrhizae and host plants using the primers ITS4LNG, ITSB, ITSML, ITSCHCH. Lanes 1 and 14: 100-bp ladder (Gibco BRL); lane 2: T. indicum ascocarp 20; lane 3: T. melanosporum ascocarp; lane 4: T. brumale ascocarp; lane 5: oak root; lane 6: hazel root; lane 7: T. indicum mycorrhiza; lane 8: T. melanosporum mycorrhiza; lane 9: T. brumale mycorrhiza; lanes 10–12: mixtures of T. indicum, T. melanosporum and T. brumale mycorrhizae at a ratio of 1:1:1 with 3 (lane 10), 9 (lane 11) and 30 (lane 12) root tips; lane 13: negative control (no DNA template).