Comparable entry factor use by pseudotypes bearing glycoprotein (GP) from ebolavirus (EBOV) strain Mayinga from 1976 (EBOV-GP 1976) and EBOV variant Makona from 2014 (EBOV-GP 2014). A, 293T cells transfected to express the indicated lectins or transfected with empty plasmid (pcDNA3) were transduced with infectivity-normalized pseudotypes bearing the indicated viral GPs. Seventy-two hours after transduction, luciferase activities were determined in cell lysates. The results of a single, representative experiment performed with triplicate samples are shown. Transduction of control transfected cells (pcDNA) was set as 1. Similar results were obtained in 5 independent experiments, using different pseudotype preparations. B, Huh7 cells (left panel) and HeLa cells (right panel) underwent small interfering RNA (siRNA) transfection and were subsequently transduced with infectivity-normalized pseudotypes. Luciferase activities in cell lysates were measured 72 hours after transduction. The experiment was performed with triplicate samples and is representative of 3 independent experiments. C, 293T cells were preincubated with solvent (dimethyl sulfoxide [DMSO]) or the respective cationic amphiphiles in the indicated concentrations and subsequently transduced with pseudotypes bearing the indicated GPs. Luciferase activities were measured 72 hours after transduction. Two additional experiments yielded comparable results. Error bars indicate standard deviations.