Gene expression profiles of F3′H and CH3H in five different Cosmos tissues: seedlings (1), young leaves (2), old leaves (3), buds (4), and flowers (5). (A) Displayed via a logarithmic y-axis. Transcript levels were quantified using plasmids harbouring a copy of the respective cDNA clone. The number of F3′H and CH3H transcripts and the ratio F3′H:CH3H are also shown. (B) qPCR assays run in 1.5% agarose gel, left, F3′H; right, CH3H.