Southern blot analysis. Southern blot analysis using the NcoI–BamHI fragments of FLON genes as probes. Lane 1: strain YN79 genome hybridized with the FLONS probe. Lane 2: FLONL hybridized with the FLONS probe. Lane 3: strain YS59 genome hybridized with the FLONS probe. Lane 4: strain YN79 genome hybridized the FLONL probe. Lane 5: strain YS58 genome hybridized with both of FLON genes. Lane 6: strain YS59 genome hybridized with the FLONL probe.