Occurrence of substitutions from predicted ancestral amino acids (on the left of matrices) to extant residues (at bottom of matrices) in halophilic protists at fastest-evolving sites of the Mandor superalignment. Amino acids are ordered from most hydrophilic to most hydrophobic. Each square is colored according to Z -score where blue represents substitutions that occurred less often in halophiles compared with the other taxa and red represents substitutions that occurred more often in halophiles. Boxed squares refer to Z -scores with P < 0.05 (in black P values corrected for multiple testing, in orange P values that were significant prior to correction).