EMSA of γMYB1 and γMYB2 binding to PLA 2 -γ? promoter fragments. (A) Diagram of P1–P6 fragments of the PLA 2 -γ promoter used for the EMSA. ProPLA 2 -γP6 is divided into five (a–e) fragments. The positions of individual fragments from the translational start codon are shown as numbers above or below the line. (B) EMSA with γMYB1 performed with 32 P-end-labeled promoter fragments P4 and P6 . The free probe of P4 was run out of the gel (C) EMSA of γMYB1 or γMYB2 with segments of P6 . The biotin-labeled probes were mixed with MYB and CC-domain proteins of either γMYB1 or γMYB2. (D) EMSA of γMYB1 or γMYB2 with segment P6_b with a specific probe competitor (non-labeled probe). (E) EMSA of γMYB1 or γMYB2 with mutated P6_b ( P6_mb ). Black arrows indicate the shifted bands; white arrows indicate reduced intensity of the shifted bands; white triangles indicate non-specific bands; a white star indicates the free probe of P6 .
