Histochemical GUS staining and fluorometric assay of the PLA 2 -γ promoter. (A) Diagram of the PLA 2 -δ and PLA 2 -γ genomic region. The gray dotted lines indicate the untranslated regions, the black boxes indicate the exons, and black bold lines indicate the introns. (B) Diagram of the PLA 2 -γ promoter and its 5′-deletion series. Numbers indicate the distance in nucleotides from the translational start codon. (C) Histochemical GUS staining of flowers of three independent T 3 homozygous Arabidopsis lines transformed with ProPLA 2 -γ : GUS or its deletion constructs. Flowers in this figure display the representative phenotypes of each line. (D) Fluorometric GUS activity assay of flowers in three independent T 3 transgenic lines. Flowers of a wild-type plant were used as a negative control. Error bars show ± SD ( n = 3).
