Lipistase confers atheroprotection in APOEKO mice. (A) Graph represents area of atherosclerotic lesions in aortic roots from mice treated with lipistase (10 mice) or vehicle (Control, 8 mice) starting at 10 weeks of age for 10 months. (B) The graph illustrates atherosclerotic plaque area in aortic roots (lipistase, eight animals; control, eight animals) from mice treated with lipistase or vehicle (Control) starting at 3 weeks of age for 3 months. (C) The graph shows plaque area in aortic roots (15 animals) from offspring of lipistase- or vehicle- (control) treated mice. These offsprings were also treated after weaning for 3 months. (D) Anti-VCAM1, anti-CD68, and anti-αSMA immunostaining (red) of aortic roots from the offspring of lipistase- or vehicle-treated mice (DAPI, blue) (left panels) with corresponding quantification (graphs, right panels). Samples from the same animals as in (C) are shown (bars = 200µm) (ns, not significant; *P<0.05; **P<0.01; ***P<0.001).