Activated platelets from infarct-related coronary artery are responsible for neutrophil extracellular trap generation. Fluorescence-activated cell-sorting analysis of Annexin V (A) and CD62P (B) on infarct-related coronary artery platelets, non-infarct-related coronary artery platelets and control individual coronary artery platelets. (C) Platelet/polymorphonuclear neutrophil aggregates observed as double-positive CD61/CD66b per 10 000 CD66b positive events with fluorescence-activated cell-sorting analysis in polymorphonuclear neutrophils isolated after selective coronary sampling. (D) Percentage of neutrophil extracellular trap-releasing polymorphonuclear neutrophils and (E) Neutrophil extracellular trap generation by control polymorphonuclear neutrophils treated with platelets isolated after selective coronary sampling, (confocal microscopy). Green: tissue factor; red: NE; blue: 4′,6-diamidino-2-phenylindole/deoxyribonucleic acid. Original magnification: ×600. Scale bar: 5 μm. (F) myeloperoxidase–deoxyribonucleic acid complex in isolated neutrophil extracellular trap structures of control polymorphonuclear neutrophils treated with platelets obtained by coronary sampling. (A), (B), (C), (D), (F) n = 4. (E) One representative out of four independent experiments is shown.