Comparison of enrichment of HNF3A/HNF3B targets between the TFBSs in the conserved and nonconserved regions on a part of the human chromosome 22. ChIP–chip analysis was performed by using an Affymetrix tiling array (GeneChip Chromosome 21/22 1.0 Array Set, c-chip). A total of 33 sites that exhibited a P-value <10^–4 were detected and they were divided in two groups according to their localization within conserved (19 sites) and nonconserved (14 sites) regions. The conserved and nonconserved regions between human and mouse genome were defined by the analysis with zPicture program, an option of the ECR browser. The enrichment of each target site was assessed by X-ChIP/qPCR with specific primer sets measuring ΔC_T, the difference between the threshold cycles observed with input chromatin DNA (1 ng) and with ChIP sample recovered by using anti-HNF3 antibody recognizing HNF3A and HNF3B. The median, upper and lower quartiles and minimum and maximum values of ΔC_T are shown in the boxplot. The outlier value is plotted with a small open circle.