Figure 9.
(A) HPLC analysis of cross-link formation in the triplex DNA. The reaction was done using ODN3 (30 μM) and DNA1 and 2 (10 μM each) in 50 mM MES buffer (pH 5.0), including 100 mM NaCl and 10 mM MgCl2 at 25°C for 1 h (B) Expected complex structures in the triplex DNA between a protonated C and a GC pair (22) and that between a protonated 2 and an AT pair (23). MMPP used for preparation of ODN3 appeared in HPLC chart.
