Figure 8.
![Evaluation of triplex cross-linking. Cross-linking conditions, [ODN3 or ODN6] = 25 µM, [DNA1/DNA2] = 5 µM, 50 mM MES buffer, 100 mM NaCl, 10 mM MgCl2, 25°C, pH 5.0 (C = dC) or pH 6.1 (C = dmC). (A) Electrophoresis, 15% denatured polyacrylamide gel. The reaction was checked at 0, 5, 15, 30 and 60 min. (B) The yield of the cross-linked product was obtained using FAM-labeled DNA1. (C) The yield was obtained using FAM-labeled DNA2.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/41/13/10.1093_nar_gkt197/3/gkt197f8p.jpeg?Expires=1749904850&Signature=TSKauek~PGG0L9tjAwVvWoBUjsJQTeHuL05DrCF6FwMmrEyw7QWkvtdlUee6~bvkhEHwqcd~rr~Fb~3jN~4dPcU-F12wnWb0vKjRaq1DTNsSoZOXTusOAY0I3t4usvslzm2j-YYhJnNQLFV4K67BsvNJHTy8Iwfb9WAHR7NHCw6N1JnpYaBt9QUW2KQP4BUeD96gslaGMIVzowyhxBq~JewdyGgIecdWdDcXx-4iPA3BR1~Pw6uUvA2fOamv-Xy3O3bsbY1s3D30YUhk22wRS8SnxbPJN7dt7RdOKk1EGZ4NKUdPYffB0ukjmdiydzqgmo9xqL7DkYABlhZ398LqZw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Evaluation of triplex cross-linking. Cross-linking conditions, [ODN3 or ODN6] = 25 µM, [DNA1/DNA2] = 5 µM, 50 mM MES buffer, 100 mM NaCl, 10 mM MgCl2, 25°C, pH 5.0 (C = dC) or pH 6.1 (C = dmC). (A) Electrophoresis, 15% denatured polyacrylamide gel. The reaction was checked at 0, 5, 15, 30 and 60 min. (B) The yield of the cross-linked product was obtained using FAM-labeled DNA1. (C) The yield was obtained using FAM-labeled DNA2.
