Figure 4.
![HPLC trace of the cross-linking reaction using ODN3 and non-labeled RNA1 (X = U). Cross-linking conditions: [ODN3] = 15 µM, RNA1 = 10 mM, 50 mM MES buffer, 100 mM NaCl, 37°C, pH 5 or pH 7. The reaction at pH9 was performed in 50 mM carbonate buffer. HPLC conditions: column SHISEIDO C18, 4.6 × 250 mm; solvent A 0.1 M TEAA buffer, solvent B CH3CN, B 10–20% /20 min 20–100%/25 min, linear gradient; flow rate 1.0 ml/min; UV 254 nm. Each peak was assigned to the A- or U-cross-link, as described in the text. MMPP used for preparation of ODN3 appeared in HPLC chart.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/41/13/10.1093_nar_gkt197/3/gkt197f4p.jpeg?Expires=1749928790&Signature=Imdgq3E-OSpKxpjxSk04IZX6saxY46szEymH7wNcmz1nywKKEjlvv2pYDeoX~WOihzpJadZV~IJ7kedA7zWX53FVB3AYDLv1KYpSsp0iBP0BFUsJyV1ErV~JW0sTg4--2-imbtJWCBh2bHBMR~LS6rbPEqGOVH-avCsqc1rTSXWnuOu-TicdtVH7Xx76fqxP8oa3RjtmLLn5ouQnSfIvr9m4eW1-QQbxJ2PHHOjpSDcBkEOZc0LEHXtt-tXmQqkQDUc0x3SH~otDr2Y~DYuTbp6buvXHDksLG~ni9GX26wUEBSVGEhXHD4mwGVtGJNbO180TJpHMdTUcUd6nJMC0Uw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HPLC trace of the cross-linking reaction using ODN3 and non-labeled RNA1 (X = U). Cross-linking conditions: [ODN3] = 15 µM, RNA1 = 10 mM, 50 mM MES buffer, 100 mM NaCl, 37°C, pH 5 or pH 7. The reaction at pH9 was performed in 50 mM carbonate buffer. HPLC conditions: column SHISEIDO C18, 4.6 × 250 mm; solvent A 0.1 M TEAA buffer, solvent B CH3CN, B 10–20% /20 min 20–100%/25 min, linear gradient; flow rate 1.0 ml/min; UV 254 nm. Each peak was assigned to the A- or U-cross-link, as described in the text. MMPP used for preparation of ODN3 appeared in HPLC chart.
