Figure 2.
![Cross-linking reaction with ODN3,4 and RNA1. Cross-linking conditions: [ODN3 or 4] = 10 µM, RNA1 = 1 µM, 50 mM MES buffer, 100 mM NaCl, 37°C, pH 7.0. The reaction was followed by electrophoresis using 15% denatured polyacrylamide gel. The fast- and slow-moving bands represent RNA1 and the cross-linked products, respectively. The cross-link yield was obtained by quantification of the bands by FAM fluorescence (λem = 518 nm, λex = 494 nm), and it was plotted against time. (A) Yield obtained with ODN3 (R = CH3). (B) Yield obtained with ODN4 (R = H). (C) An example of gel-shift analysis of the reaction with ODN3 (R = CH3).](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/41/13/10.1093_nar_gkt197/3/gkt197f2p.jpeg?Expires=1750232104&Signature=CmKqem9V6k9o2qsJLxtvrJUlQS4nidCBKp~~9fzY9x~brqM-aXIz9~WGNlLOq9nVYcPS7vhQryF348ZLhjzw-ODw~bczcCho~AylK~zn~0FTXNXjqDMscP1VKjUMA7XiZ~e1waAwp5B8lK0OZmDh4Q1rFSbOYd3ucHudvgoxCEQMIA70nqKpbWs7dMMkQnCs9qs9arLSk79YTxd~lPAd-Ou8B7Ndh~PlqPywQTIvn0Ii5l8UJHM4LY-vuvDzoEHYz6ShiZQaZ43ZcmrD7Ir3LqDgpWnSFT9pmrIxJ~H0qFnYn9cwh1tsfcSs8~ezvsnWO7Gd2E9tW06~RFMS~uj3Fg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cross-linking reaction with ODN3,4 and RNA1. Cross-linking conditions: [ODN3 or 4] = 10 µM, RNA1 = 1 µM, 50 mM MES buffer, 100 mM NaCl, 37°C, pH 7.0. The reaction was followed by electrophoresis using 15% denatured polyacrylamide gel. The fast- and slow-moving bands represent RNA1 and the cross-linked products, respectively. The cross-link yield was obtained by quantification of the bands by FAM fluorescence (λem = 518 nm, λex = 494 nm), and it was plotted against time. (A) Yield obtained with ODN3 (R = CH3). (B) Yield obtained with ODN4 (R = H). (C) An example of gel-shift analysis of the reaction with ODN3 (R = CH3).
