RT-PCR analysis of expression levels of two sHSP genes and one senescence gene (LePLDa1) of 5-day fruit from HT and HS lines exposed to the control temperature (25/20 °C, day/night) and after 24-h strong heat stress (36/28 °C, day/night). The sHSP genes, LeHsp 17.4-CII and LeHsp 17.6-CII, were amplified by 30 cycles and the senescence gene, LePLDa1, was amplified by 25 cycles. The reference gene, Actin, was amplified at 30 cycles.