Detection of AtMago, AtY14, and AtPYM in wild-type and overexpressing plants. Extracts from wild-type plants and from plants transformed with constructs that express AtMago, AtY14, or AtPYM under the control of the 35SCaMV promoter were analysed by non-denaturing (A, B) or denaturing (C) PAGE followed by western blots using specific antibodies (indicated beside each panel). In B, the extract was treated with different amounts of alkaline phosphatase before the analysis.