(A) Schematic diagram of the genome of HCV JFH-1 (top) and Con1b-luc-rep (below) indicating the location of RNA stem–loop structures (SL) using both standardised positional references and naming schemes from previous publications. (B) Schematic representation of the dynamic SL9266/PK pseudoknot showing its open and closed conformations. A dashed line represents genome regions that switch between alternative conformations. (C) Schematic of representation of SL9266 and SL9571 in Con1b showing individual nucleotides labelled with standardised positional references and locations of the kissing loop (PK) and upstream interactions labelled in reverse camera. Dashed lines represents sequence regions not shown. (D) Table shows schematic representations of SL9266/PK with antisense-LNA binding sites represented by red lines, non-specific linkers are shown as dashed red lines. LNA-oligonucleotides are named for the upstream nucleotide to which they are predicted to bind and are preceded by a C (for Con1b) or J (for JFH-1)—depending on which HCV isolate they target.