The CYP1A1–CYP1A2 intergenic region that was used to validate a large-scale PCRTiler result. PCRTiler was used to compute primer pairs in this region, designing one primer pair every 200 bp. The intergenic region shown measures 24.3 kb. Each vertical bar (123) represents an amplicon targeted by a primer pair designed by PCRTiler. A closeup view of the first 10 amplicons is shown underneath.